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APRIL 24
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– 26
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, 2014 | OLOMOUC | THE CZECH REPUBLIC
ABS TRAC T BOOK
EMT (epithelial-to-mesenchymal transition) and epithelial from
MET (mesenchymal-to-epithelial transition). To understand the
relationship between EMT, MET and BCSC we used HER2/neu-
-overexpressing primary mouse mammary carcinoma cell line
(MMC) and its relapsed HER2/neu antigen-negative variants
(ANV) [2]. Analysis at the single cell level revealed different
expression of surface stem cell markers (CD44, CD49f, CD133,
Trop-2 and Sca-1), transcription factors responsible for stemness
(Oct-3/4, Sox-2, Nanog) and EMT/MET regulators (Snail, Slug, Axl)
amongst cell lines. We examined basal expression of epithelial
and mesenchymal markers and also EMT regulators in various
BCSC populations and expression after induced switch of
phenotype via different signaling pathways. De-differentiation
of ANV clones during the EMT transformation, accompanied
by acquisition of Sca-1 expression, confers that the stemness
and EMT might be related in mouse mammary carcinoma cells
and their relapsed variants.
The authors would like to thank Dr. Keith L. Knutson for
providing model cell lines. This work was supported by grants
Histopark no. CZ.1.07/2.3.00/20.0185 and OrganoNET no.
CZ.1.07/2.4.00/31.0245 of Ministry of Education, Youth and Sports,
IGA MZD NT13573-4/2012, AV ČR M200041203, and by project
FNUSA-ICRC (no. CZ.1.05/1.1.00/02.0123) from the European
Regional Development Fund. Institutional support was provided
by the Academy of Sciences of the Czech Republic.
References
1.
Suling L, et al. Breast Cancer Stem Cells Transition between Epithelial and Mesenchy-
mal States Reflective of their Normal Counterparts. Stem Cell Reports, 2014.
2.
Knutson KL, et al. neu Antigen-Negative Variants Can Be Generated after neu-Speci-
fic Antipody Therapy in neu Transgenic Mice. Cancer Research, 2004.
