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ABS TRAC T BOOK
their projections are in very tight relationship to the capillary
wall and demarcate stromal channels in normal villi. Both the
hypo- and hypervascularized villi are very poor in those cells.
Our findings make evident that the abnormal forms of
placental terminal villi may change conditions of the mater-
nal blood circulation in their neighborhood, that a kind of
disturbed angiogenesis produces abnormal villous capillary
bed, that the cells of such villi display decreased proliferation
potential, and that their stromal architecture is disrupted. The
presented results suggest that higher ratio of those villi may
impair placental function and thus threaten fetal well being.
Supported by PRVOUK P25/LF1/2.
5) CTHRC1, periostin and versican in the
progression of prostate cancer
Kharaishvili G., Bouchal J., Kral M., Kolar Z.
Department of Clinical and Molecular Pathology,
Laboratory of Molecular Pathology, Institute of Molecular
and Translational Medicine, Palacky University and
University Hospital, Olomouc
Background:
We have previously reported collagen triple
helix repeat containing 1 (CTHRC1) as breast cancer related
protein (Turashvili et al 2007) and its potent role in breast cancer
bone metastases in combination with periostin (Kharaishvili
et al 2011). CTHRC1 has been shown to affect Wnt signaling,
collagen deposition and bone formation and generally belongs
to extracellular matrix proteins which are abnormally expressed
in tumor microenvironment. CTHRC1 has not been studied in
prostate cancer yet. We decided to verify its expression in our
patients set together with periostin and versican and other
relevant proteins.
Material and methods:
Formalin fixed paraffin embed-
ded tissues of 101 prostate carcinomas (PCa) and 32 benign
(BPH) cases were stained immunohistochemically for CTHRC1,
periostin, versican, E-cadherin and beta-catenin, and scored.
Carcinomas were classified into localized and advanced groups.
Statistical analysis was performed by SPSS software.
Results:
CTHRC1 was predominantly localized in cancer
cell cytoplasm and extracellular matrix, while versican and
periostin were rarely positive in cancer cells. All three proteins
were expressed in the stroma surrounding cancer. Expression
of all markers was significantly higher in PCa in comparison
to BPH (CTHRC1 in cancer epithelium and stroma, periostin
in stroma, all p<0.001, versican in stroma p=0.036), and also
higher in advanced cases in comparison to localized ones
(CTHRC1 epithelial p=0.002; periostin stromal p=0.001; versican
stromal p<0.001).
CTHRC1 stromal and epithelial expression significantly
correlated with periostin stomal positivity (r=0.232, p=0.008
and r=0.235, p=0.007, respectively). Versican and periostin
stromal expressions were also in association (r=0.438, p<0.001).
These two proteins showed similar tissue expression patterns.
Periostin inversely correlated with membranous beta-catenin
and E-cadherin (r=-0.4 and -0.367, respectively, both p<0.001).
CTHRC1 also showed negative correlation with E-cadherin
(r=0.188, p=0.03).
Conclusions:
We showed that CHTRC1, periostin and ver-
sican are prostate cancer related proteins and are expressed
in cancer cells and epithelial-mesenchymal interface. In the
performed study their significant increase in prostate cancer
tissue demonstrate that mentioned proteins play a role in the
progression of prostate cancer.
Acknowledgements: The study was supported in part by
grant NT13573 from the Czech Ministry of Health and EU infra-
structure support CZ.1.05/2.1.00/01.0030.
6) Immunorectivity in the cardiac tissue of the
crocodile embryos using conventional antibodies
Kvasilova A., Sedmera D.
1
Institute of Anatomy, Charles University, Prague,
Czech Republic
2
Institute of Physiology, Academy of Sciences of the Czech
Republic
According to phylogeny the crocodile has the most perfect
heart of all the reptiles, having a completed septation of the
ventricles similar to the human heart. In the basic features it is
thus very similar to human. However, the question of presence
or nature of the crocodilian cardiac conduction system is not
yet clarified. To be able to embark upon the study of this issue,
we attempted to identify working antibodies to crocodile
tissue from commercially available antibodies. We used the
antibodies, which are known to react with mouse, rat, human
or chicken, as antibodies tested specifically for crocodile tissues
are currently not available.
Experiments were carried out on a series of crocodile em-
bryonic hearts of Crocodylus siamensis at different stages of
development. We dissected the thorax and opened the chest
cavity to gain the access to the heart. The hearts were isolated
and fixed for 24 h at 4°C in 4% paraformaldehyde in PBS and
then processed into OCT medium for serial sectioning on a
cryomicrotome. We first performed H&E / Alcian Blue staining
in histological sections at 100 µm intervals (guide series). Then
we selected slices containing the tissues of interest, where we
focused primarily on the atria, the interventricular septum,
the ventricles, and the outflow tract. We tested the following
antibodies for immunostaining: α-Smooth Muscle Actin (SMA),
α-Sarcomeric Actin (SA), Collagen type I, Periostin, Connexin
40, HCN4 and CD57 (HNK-1). In parallel with crocodile tissue
we processed control chick and/or mouse embryonic heart
samples, which served as a positive control.
Immunohistochemistry using SMA, SA, Periostin and CD57
antibodies in crocodile tissues produced expected results si-
milar to control samples. Interestingly, SMA staining persisted
in the crocodile ventricles even after septation, while it was
absent in older chick or mouse hearts. The other antibodies
produced either non-specific staining, or there was no immu-
